Review



mouse anti lasv np mabs  (Zalgen Labs)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Zalgen Labs mouse anti lasv np mabs
    A) Cross-reactivity <t>of</t> <t>mAbs</t> to different <t>LASV</t> lineages NP was measured using cell lysate in indirect ELISA. OD 450 for each mAb is summarized as heat map. B) Representative of binding of anti-LASV NP mAb with cell lysate of HEK293T expressing LASV NPs (lineage I-VII) using WB, each lysate was diluted 1:100 times and used as an antigen for measurement of binding of mAb 28-2-3. C) Representative images of IFA showing binding of mAb 57-2 to the HEK293T cells expressing LASV NP, 24 hours post transfection cells were fixed, permeabilized and incubated with mAb 57-2 and detected by Alexa488 anti-mouse IgG antibody. Hoechst was used for nucleus staining. HEK293T cells transfected with pCAGGS empty vector were used as negative control (NC).
    Mouse Anti Lasv Np Mabs, supplied by Zalgen Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+lasv+np+mabs/12+1f+anti+gp1+human+lasv/pmc13175458-102-8-21
    Average 86 stars, based on 1 article reviews
    mouse anti lasv np mabs - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Development of novel monoclonal antibodies for detection of pan- Lassa virus"

    Article Title: Development of novel monoclonal antibodies for detection of pan- Lassa virus

    Journal: PLOS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0014326

    A) Cross-reactivity of mAbs to different LASV lineages NP was measured using cell lysate in indirect ELISA. OD 450 for each mAb is summarized as heat map. B) Representative of binding of anti-LASV NP mAb with cell lysate of HEK293T expressing LASV NPs (lineage I-VII) using WB, each lysate was diluted 1:100 times and used as an antigen for measurement of binding of mAb 28-2-3. C) Representative images of IFA showing binding of mAb 57-2 to the HEK293T cells expressing LASV NP, 24 hours post transfection cells were fixed, permeabilized and incubated with mAb 57-2 and detected by Alexa488 anti-mouse IgG antibody. Hoechst was used for nucleus staining. HEK293T cells transfected with pCAGGS empty vector were used as negative control (NC).
    Figure Legend Snippet: A) Cross-reactivity of mAbs to different LASV lineages NP was measured using cell lysate in indirect ELISA. OD 450 for each mAb is summarized as heat map. B) Representative of binding of anti-LASV NP mAb with cell lysate of HEK293T expressing LASV NPs (lineage I-VII) using WB, each lysate was diluted 1:100 times and used as an antigen for measurement of binding of mAb 28-2-3. C) Representative images of IFA showing binding of mAb 57-2 to the HEK293T cells expressing LASV NP, 24 hours post transfection cells were fixed, permeabilized and incubated with mAb 57-2 and detected by Alexa488 anti-mouse IgG antibody. Hoechst was used for nucleus staining. HEK293T cells transfected with pCAGGS empty vector were used as negative control (NC).

    Techniques Used: Indirect ELISA, Binding Assay, Expressing, Transfection, Incubation, Staining, Plasmid Preparation, Negative Control

    The cross-competition profile of mAbs for binding to LASV NP was determined using Ab competitive ELISA. HEK293T cell lysate expressing LASV lineage III (Ojoko) NP was diluted to 1:100 in PBS and coated on the ELISA plate surface. Serially diluted unconjugated mAbs were used as primary antibody as represented in different colors in each graph and then 1µg/ml of HRP-conjugated mAbs for LASV NP were used as secondary antibody for each measurement. Anti-LASV GPC mAb was used as an IgG control.
    Figure Legend Snippet: The cross-competition profile of mAbs for binding to LASV NP was determined using Ab competitive ELISA. HEK293T cell lysate expressing LASV lineage III (Ojoko) NP was diluted to 1:100 in PBS and coated on the ELISA plate surface. Serially diluted unconjugated mAbs were used as primary antibody as represented in different colors in each graph and then 1µg/ml of HRP-conjugated mAbs for LASV NP were used as secondary antibody for each measurement. Anti-LASV GPC mAb was used as an IgG control.

    Techniques Used: Binding Assay, Competitive ELISA, Expressing, Enzyme-linked Immunosorbent Assay, Control

    A) 2x10 5 PFU/ml of LASV lineages I-IV were serially diluted and used as an antigen for sandwich ELISA with 1µg/ml of each capturing and detection mAbs. Binding of different pairs of capturing mAb/detection mAb with LASV NP are denoted in the graph as OD 450 . B) Sandwich ELISA for detection of LASV NP from lineages V-VII were measured using VLPs, lineage IV VLPs was used as positive control for this measurement. C) Measurement of NP mAbs binding with rVSV, EBOV, MARV, and LCMV at different concentration of authentic virus using different combinations of mAbs. Data represents means and standard deviations (SDs) of OD 450 from three biological triplicates. The threshold of sandwich ELISA was calculated as the mean + 3SDs of OD 450 value of negative control and represented as a black dotted horizontal line in the graphs.
    Figure Legend Snippet: A) 2x10 5 PFU/ml of LASV lineages I-IV were serially diluted and used as an antigen for sandwich ELISA with 1µg/ml of each capturing and detection mAbs. Binding of different pairs of capturing mAb/detection mAb with LASV NP are denoted in the graph as OD 450 . B) Sandwich ELISA for detection of LASV NP from lineages V-VII were measured using VLPs, lineage IV VLPs was used as positive control for this measurement. C) Measurement of NP mAbs binding with rVSV, EBOV, MARV, and LCMV at different concentration of authentic virus using different combinations of mAbs. Data represents means and standard deviations (SDs) of OD 450 from three biological triplicates. The threshold of sandwich ELISA was calculated as the mean + 3SDs of OD 450 value of negative control and represented as a black dotted horizontal line in the graphs.

    Techniques Used: Sandwich ELISA, Binding Assay, Positive Control, Concentration Assay, Virus, Negative Control

    Related Articles

    Membrane:

    Article Title: Development of novel monoclonal antibodies for detection of pan- Lassa virus
    Article Snippet: Subsequently, the separated proteins were transferred to a polyvinylidene fluoride (PVDF) membranes (Merck Millipore) and blocked for 1 hour using PBS supplemented with 0.05% Tween 20 (PBST) containing 5% skimmed milk. .. The membrane was incubated with 1 μg/ml of mouse anti-LASV NP mAbs which were generated in house or commercial mAb from Zalgen (NP LASV MAb1474) was also used to detect LASV NP in the cell lysate. .. After washing three times with PBST, the membrane was incubated for 1 hour with horseradish peroxidase (HRP)-conjugated Goat anti-Mouse IgG (H + L) (1:10,000, Invitrogen) or Anti-rabbit IgG, HRP-linked Antibody (1:10,000, cell signaling technology), and protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and images of blots were captured using Azure 300 (Azure biosystem).

    Incubation:

    Article Title: Development of novel monoclonal antibodies for detection of pan- Lassa virus
    Article Snippet: Subsequently, the separated proteins were transferred to a polyvinylidene fluoride (PVDF) membranes (Merck Millipore) and blocked for 1 hour using PBS supplemented with 0.05% Tween 20 (PBST) containing 5% skimmed milk. .. The membrane was incubated with 1 μg/ml of mouse anti-LASV NP mAbs which were generated in house or commercial mAb from Zalgen (NP LASV MAb1474) was also used to detect LASV NP in the cell lysate. .. After washing three times with PBST, the membrane was incubated for 1 hour with horseradish peroxidase (HRP)-conjugated Goat anti-Mouse IgG (H + L) (1:10,000, Invitrogen) or Anti-rabbit IgG, HRP-linked Antibody (1:10,000, cell signaling technology), and protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and images of blots were captured using Azure 300 (Azure biosystem).

    Generated:

    Article Title: Development of novel monoclonal antibodies for detection of pan- Lassa virus
    Article Snippet: Subsequently, the separated proteins were transferred to a polyvinylidene fluoride (PVDF) membranes (Merck Millipore) and blocked for 1 hour using PBS supplemented with 0.05% Tween 20 (PBST) containing 5% skimmed milk. .. The membrane was incubated with 1 μg/ml of mouse anti-LASV NP mAbs which were generated in house or commercial mAb from Zalgen (NP LASV MAb1474) was also used to detect LASV NP in the cell lysate. .. After washing three times with PBST, the membrane was incubated for 1 hour with horseradish peroxidase (HRP)-conjugated Goat anti-Mouse IgG (H + L) (1:10,000, Invitrogen) or Anti-rabbit IgG, HRP-linked Antibody (1:10,000, cell signaling technology), and protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and images of blots were captured using Azure 300 (Azure biosystem).



    Similar Products

    mouse anti lasv np mabs  (Zalgen Labs)
    86
    Zalgen Labs mouse anti lasv np mabs
    A) Cross-reactivity <t>of</t> <t>mAbs</t> to different <t>LASV</t> lineages NP was measured using cell lysate in indirect ELISA. OD 450 for each mAb is summarized as heat map. B) Representative of binding of anti-LASV NP mAb with cell lysate of HEK293T expressing LASV NPs (lineage I-VII) using WB, each lysate was diluted 1:100 times and used as an antigen for measurement of binding of mAb 28-2-3. C) Representative images of IFA showing binding of mAb 57-2 to the HEK293T cells expressing LASV NP, 24 hours post transfection cells were fixed, permeabilized and incubated with mAb 57-2 and detected by Alexa488 anti-mouse IgG antibody. Hoechst was used for nucleus staining. HEK293T cells transfected with pCAGGS empty vector were used as negative control (NC).
    Mouse Anti Lasv Np Mabs, supplied by Zalgen Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+lasv+np+mabs/12+1f+anti+gp1+human+lasv/pmc13175458-102-8-21
    Average 86 stars, based on 1 article reviews
    mouse anti lasv np mabs - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Cross-reactivity of mAbs to different LASV lineages NP was measured using cell lysate in indirect ELISA. OD 450 for each mAb is summarized as heat map. B) Representative of binding of anti-LASV NP mAb with cell lysate of HEK293T expressing LASV NPs (lineage I-VII) using WB, each lysate was diluted 1:100 times and used as an antigen for measurement of binding of mAb 28-2-3. C) Representative images of IFA showing binding of mAb 57-2 to the HEK293T cells expressing LASV NP, 24 hours post transfection cells were fixed, permeabilized and incubated with mAb 57-2 and detected by Alexa488 anti-mouse IgG antibody. Hoechst was used for nucleus staining. HEK293T cells transfected with pCAGGS empty vector were used as negative control (NC).

    Journal: PLOS Neglected Tropical Diseases

    Article Title: Development of novel monoclonal antibodies for detection of pan- Lassa virus

    doi: 10.1371/journal.pntd.0014326

    Figure Lengend Snippet: A) Cross-reactivity of mAbs to different LASV lineages NP was measured using cell lysate in indirect ELISA. OD 450 for each mAb is summarized as heat map. B) Representative of binding of anti-LASV NP mAb with cell lysate of HEK293T expressing LASV NPs (lineage I-VII) using WB, each lysate was diluted 1:100 times and used as an antigen for measurement of binding of mAb 28-2-3. C) Representative images of IFA showing binding of mAb 57-2 to the HEK293T cells expressing LASV NP, 24 hours post transfection cells were fixed, permeabilized and incubated with mAb 57-2 and detected by Alexa488 anti-mouse IgG antibody. Hoechst was used for nucleus staining. HEK293T cells transfected with pCAGGS empty vector were used as negative control (NC).

    Article Snippet: The membrane was incubated with 1 μg/ml of mouse anti-LASV NP mAbs which were generated in house or commercial mAb from Zalgen (NP LASV MAb1474) was also used to detect LASV NP in the cell lysate.

    Techniques: Indirect ELISA, Binding Assay, Expressing, Transfection, Incubation, Staining, Plasmid Preparation, Negative Control

    The cross-competition profile of mAbs for binding to LASV NP was determined using Ab competitive ELISA. HEK293T cell lysate expressing LASV lineage III (Ojoko) NP was diluted to 1:100 in PBS and coated on the ELISA plate surface. Serially diluted unconjugated mAbs were used as primary antibody as represented in different colors in each graph and then 1µg/ml of HRP-conjugated mAbs for LASV NP were used as secondary antibody for each measurement. Anti-LASV GPC mAb was used as an IgG control.

    Journal: PLOS Neglected Tropical Diseases

    Article Title: Development of novel monoclonal antibodies for detection of pan- Lassa virus

    doi: 10.1371/journal.pntd.0014326

    Figure Lengend Snippet: The cross-competition profile of mAbs for binding to LASV NP was determined using Ab competitive ELISA. HEK293T cell lysate expressing LASV lineage III (Ojoko) NP was diluted to 1:100 in PBS and coated on the ELISA plate surface. Serially diluted unconjugated mAbs were used as primary antibody as represented in different colors in each graph and then 1µg/ml of HRP-conjugated mAbs for LASV NP were used as secondary antibody for each measurement. Anti-LASV GPC mAb was used as an IgG control.

    Article Snippet: The membrane was incubated with 1 μg/ml of mouse anti-LASV NP mAbs which were generated in house or commercial mAb from Zalgen (NP LASV MAb1474) was also used to detect LASV NP in the cell lysate.

    Techniques: Binding Assay, Competitive ELISA, Expressing, Enzyme-linked Immunosorbent Assay, Control

    A) 2x10 5 PFU/ml of LASV lineages I-IV were serially diluted and used as an antigen for sandwich ELISA with 1µg/ml of each capturing and detection mAbs. Binding of different pairs of capturing mAb/detection mAb with LASV NP are denoted in the graph as OD 450 . B) Sandwich ELISA for detection of LASV NP from lineages V-VII were measured using VLPs, lineage IV VLPs was used as positive control for this measurement. C) Measurement of NP mAbs binding with rVSV, EBOV, MARV, and LCMV at different concentration of authentic virus using different combinations of mAbs. Data represents means and standard deviations (SDs) of OD 450 from three biological triplicates. The threshold of sandwich ELISA was calculated as the mean + 3SDs of OD 450 value of negative control and represented as a black dotted horizontal line in the graphs.

    Journal: PLOS Neglected Tropical Diseases

    Article Title: Development of novel monoclonal antibodies for detection of pan- Lassa virus

    doi: 10.1371/journal.pntd.0014326

    Figure Lengend Snippet: A) 2x10 5 PFU/ml of LASV lineages I-IV were serially diluted and used as an antigen for sandwich ELISA with 1µg/ml of each capturing and detection mAbs. Binding of different pairs of capturing mAb/detection mAb with LASV NP are denoted in the graph as OD 450 . B) Sandwich ELISA for detection of LASV NP from lineages V-VII were measured using VLPs, lineage IV VLPs was used as positive control for this measurement. C) Measurement of NP mAbs binding with rVSV, EBOV, MARV, and LCMV at different concentration of authentic virus using different combinations of mAbs. Data represents means and standard deviations (SDs) of OD 450 from three biological triplicates. The threshold of sandwich ELISA was calculated as the mean + 3SDs of OD 450 value of negative control and represented as a black dotted horizontal line in the graphs.

    Article Snippet: The membrane was incubated with 1 μg/ml of mouse anti-LASV NP mAbs which were generated in house or commercial mAb from Zalgen (NP LASV MAb1474) was also used to detect LASV NP in the cell lysate.

    Techniques: Sandwich ELISA, Binding Assay, Positive Control, Concentration Assay, Virus, Negative Control